伽马射线通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简述 ：阐释紫外光是否可通过升华细胞因子-β（TGF-β）-内皮细胞的内膜-间质切换 (EMT)来促进癌核酸的波及迁移。使用总额2Gy(60)Coγ线照射到源自人类器官的6种癌核酸，记录与EMT相关的变化，这包括分别透过显微镜技术，核酸印迹工具，免疫荧光技术，污垢试验和Transwell鲍洛通试验来检视并测定核酸组织形态，EMT上面，波及迁移潜能等。采用酶联成免疫吸附法测定这些癌核酸之前TGF-β蛋白总体，透过特别诱导剂SB431542来评估TGF-β频谱通路在紫外光EMT之前的作用。经过总额为2Gy照射到的癌核酸之前存在间叶核酸的表达，与假照射到组相比其内膜上面减少，间叶核酸上面增加，同时其波及转移潜能弱化，TGF-β蛋白总体也提高。实质性推测由A549紫外光诱导的EMT可通过对TGF-β频谱诱导引发逆转。这些结果显示TGF-β内皮细胞的EMT在紫外光诱导弱化癌核酸波及转移潜能之前起着关键。

编者： lizexiu